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Chicken Anti-Human IgG (H&L) secondary antibody 

  • Catalog # : PAB31778
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  • Specification
  • Product Description:
  • Chicken polyclonal antibody raised against human IgG (H&L).
  • Immunogen:
  • Human IgG whole molecule.
  • Host:
  • Chicken
  • Reactivity:
  • Human
  • Specificity:
  • IgG (H&L). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-chicken serum, human IgG and human serum.
  • Form:
  • Liquid
  • Conjugation:
  • Unconjugated
  • Purification:
  • This antibody was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (1:20000-1:60000)
    Immunohistochemistry (1:1000-1:5000)
    Western Blot (1:2000-1:10000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (0.01% (w/v) sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage, aliquot contents and freeze at -20°C or below.
    Aliquot to avoid repeated freezing and thawing.
    This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Immunohistochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • ELISA results of this antibody tested against purified human IgG. Each well was coated in duplicate with 10 ug of human IgG. The working dilution of Anti-Human IgG is 1:110,000. The starting dilution of antibody was 5 ug/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer, Goat Anti-Chicken IgG mx10 HRP conjugated and TMB substrate.
  • Application Image
  • Western Blot
  • Immunohistochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
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