Chicken Anti-Human IgG (H&L) secondary antibody
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More Files
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Enzyme-linked Immunoabsorbent Assay
ELISA results of this antibody tested against purified human IgG. Each well was coated in duplicate with 10 ug of human IgG. The working dilution of Anti-Human IgG is 1:110,000. The starting dilution of antibody was 5 ug/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer, Goat Anti-Chicken IgG mx10 HRP conjugated and TMB substrate.
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Specification
Product Description
Chicken polyclonal antibody raised against human IgG (H&L).
Immunogen
Human IgG whole molecule.
Host
Chicken
Reactivity
Human
Specificity
IgG (H&L). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-chicken serum, human IgG and human serum.
Form
Liquid
Conjugation
Unconjugated
Purification
This antibody was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Isotype
IgG
Recommend Usage
ELISA (1:20000-1:60000)
Immunohistochemistry (1:1000-1:5000)
Western Blot (1:2000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (0.01% (w/v) sodium azide).
Storage Instruction
Store at 4°C. For long term storage, aliquot contents and freeze at -20°C or below.
Aliquot to avoid repeated freezing and thawing.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
ELISA results of this antibody tested against purified human IgG. Each well was coated in duplicate with 10 ug of human IgG. The working dilution of Anti-Human IgG is 1:110,000. The starting dilution of antibody was 5 ug/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer, Goat Anti-Chicken IgG mx10 HRP conjugated and TMB substrate.
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