Western blot analysis of His-tag polyclonal antibody (1:500) and Goat Anti-Rabbit IgG (H&L) secondary antibody (HRP) (1:20000), Lane 1: 293F cell lysate, Lane 2: 293F cell transfection pcDNA3.0-ORF6-His. Western blot analysis of SARS-CoV-2 ORF6 polyclonal antibody (1:500) and Goat Anti-Rabbit IgG (H&L) secondary antibody (HRP) (1:20000), Lane 3: 293F cell transfection pcDNA3.0-ORF6-His, Lane 4: 293F cell lysate (negative control).