SARS-CoV-2 S polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: WT 293 cells and Lane 2: SARS-CoV-2 Spike overexpressed 293 cells. Loading: 15 ug per lane of 293 cell lysate. Antibodies: SARS-CoV-2 S polyclonal antibody (Cat # PAB31725) (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using SARS-CoV-2 S polyclonal antibody (Cat # PAB31725, 0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in macrophages and air epithelium of COVID-19 patient lung, but not in non-COVID-19 patient lung.
Immunofluorescence
Immunofluorescent analysis of 4% paraformaldehydefixed 293T cells labeling SARS-CoV-2 (COVID-19) Spike with Cat # PAB31725 at 1 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Enzyme-linked Immunoabsorbent Assay
A direct ELISA was performed using antigen or control peptide as coating antigen and this antibody as the capture antibody (1 ug/mL). Secondary: Goat anti-rabbit igG HRP conjugate at 1:20000 dilution. Detection range is from 0.5 to 1000 ng/mL.
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Specification
Product Description
Rabbit Polyclonal antibody raised against synthetic peptide of SARS-CoV-2 S.
Immunogen
A synthetic peptide corresponding to 20 amino acids near the C-terminus of SARS-CoV-2 Spike glycoprotein. The immunogen is located within the last 50 amino acids of SARS-CoV-2 Spike protein.
Host
Rabbit
Reactivity
SARS-CoV-2
Form
Liquid
Purification
Peptide affinity purification
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1 ug/mL)
Immunohistochemistry (0.2 ug/mL)
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide).
Storage Instruction
Store at 4°C for up to one year.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: WT 293 cells and Lane 2: SARS-CoV-2 Spike overexpressed 293 cells. Loading: 15 ug per lane of 293 cell lysate. Antibodies: SARS-CoV-2 S polyclonal antibody (Cat # PAB31725) (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP conjugate at 1:10000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using SARS-CoV-2 S polyclonal antibody (Cat # PAB31725, 0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in macrophages and air epithelium of COVID-19 patient lung, but not in non-COVID-19 patient lung.Immunofluorescence
Immunofluorescent analysis of 4% paraformaldehydefixed 293T cells labeling SARS-CoV-2 (COVID-19) Spike with Cat # PAB31725 at 1 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).Enzyme-linked Immunoabsorbent Assay
A direct ELISA was performed using antigen or control peptide as coating antigen and this antibody as the capture antibody (1 ug/mL). Secondary: Goat anti-rabbit igG HRP conjugate at 1:20000 dilution. Detection range is from 0.5 to 1000 ng/mL. -
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