STK10 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The STK10 polyclonal antibody (Cat # PAB3171) is used in Western blot to detect STK10 in Jurkat cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with STK10 polyclonal antibody (Cat # PAB3171) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of STK10.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human STK10.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The STK10 polyclonal antibody (Cat # PAB3171) is used in Western blot to detect STK10 in Jurkat cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with STK10 polyclonal antibody (Cat # PAB3171) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — STK10
Entrez GeneID
6793Protein Accession#
O94804Gene Name
STK10
Gene Alias
LOK, PRO2729
Gene Description
serine/threonine kinase 10
Omim ID
603919Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. The protein can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. The kinase can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway. [provided by RefSeq
Other Designations
lymphocyte-oriented kinase
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Interactome
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Disease
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Publication Reference
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Molecular cloning of the human gene STK10 encoding lymphocyte-oriented kinase, and comparative chromosomal mapping of the human, mouse, and rat homologues.
Kuramochi S, Matsuda Y, Okamoto M, Kitamura F, Yonekawa H, Karasuyama H.
Immunogenetics 1999 May; 49(5):369.
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Molecular cloning of the human gene STK10 encoding lymphocyte-oriented kinase, and comparative chromosomal mapping of the human, mouse, and rat homologues.
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