MAPK15 (phospho T175/Y177) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of human MAPK15 (phospho T175/Y177).
Immunogen
A synthetic peptide corresponding to amino acids 110-190 of human MAPK15 (phospho T175/Y177).
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody detects endogenous levels of MAPK15 protein only when phosphorylated at T175/Y177.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
ELISA (1:5000)
Immunohistochemistry (1:100-300)
Western Blot (1:500-2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 0.5% BSA and 0.02% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of Jurkat cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain (left). Negative control was pre-absorbed by immunogen peptide (right).Enzyme-linked Immunoabsorbent Assay
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Gene Info — MAPK15
Entrez GeneID
225689Protein Accession#
Q8TD08Gene Name
MAPK15
Gene Alias
ERK7, ERK8
Gene Description
mitogen-activated protein kinase 15
Gene Ontology
HyperlinkOther Designations
OTTHUMP00000189334|extracellular regulated kinase 8 delta|extracellular signal regulated kinase 8|extracellular signal-regulated kinase 8
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Interactome
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