MCM4 (phospho S54) polyclonal antibody
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain (left). Negative control was pre-absorbed by immunogen peptide (right).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of human MCM4 (phospho S54).
Immunogen
A synthetic peptide corresponding to amino acids 30-110 of human MCM4 (phospho S54).
Host
Rabbit
Reactivity
Human, Monkey, Mouse
Specificity
This antibody detects endogenous levels of MCM4 protein only when phosphorylated at S54.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
ELISA (1:20000)
Immunohistochemistry (1:100-300)
Western Blot (1:500-2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 0.5% BSA and 0.02% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain (left). Negative control was pre-absorbed by immunogen peptide (right).Enzyme-linked Immunoabsorbent Assay
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Gene Info — MCM4
Entrez GeneID
4173Protein Accession#
P33991Gene Name
MCM4
Gene Alias
CDC21, CDC54, MGC33310, P1-CDC21, hCdc21
Gene Description
minichromosome maintenance complex component 4
Omim ID
602638Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq
Other Designations
DNA replication licensing factor MCM4|MCM4 minichromosome maintenance deficient 4|homolog of S. pombe cell devision cycle 21|minichromosome maintenance deficient 4
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Interactome
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