UBE4B polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of (1) human cell line RT-4, and (2) human cell line U-251MG sp.
Western Blot
Western Blot analysis of (1) NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), and (2) NBT-II cell lysate (Rat Wistar bladder tumour cells).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human UBE4B.
Immunogen
Recombinant protein corresponding to amino acids 2-67 of human UBE4B.
Sequence
EELSADEIRRRRLARLAGGQTSQPTTPLTSPQRENPPGPPIAASAPGPSQSLGLNVHNMTPATSPI
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Western Blot (0.4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C for short term storage. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of (1) human cell line RT-4, and (2) human cell line U-251MG sp.Western Blot
Western Blot analysis of (1) NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), and (2) NBT-II cell lysate (Rat Wistar bladder tumour cells).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells. -
Gene Info — UBE4B
Entrez GeneID
10277Protein Accession#
O95155Gene Name
UBE4B
Gene Alias
E4, HDNB1, KIAA0684, UBOX3, UFD2
Gene Description
ubiquitination factor E4B (UFD2 homolog, yeast)
Gene Ontology
HyperlinkGene Summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000001726|OTTHUMP00000001727|homologous to yeast UFD2|homozygously deleted in neuroblastoma-1|ubiquitin-fusion degradation protein 2|ubiquitination factor E4B|ubiquitination factor E4B (homologous to yeast UFD2)
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Interactome
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Pathway
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Disease
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Publication Reference
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Proteomic screen reveals Fbw7 as a modulator of the NF-κB pathway.
Arabi A, Ullah K, Branca RM, Johansson J, Bandarra D, Haneklaus M, Fu J, Aries I, Nilsson P, Den Boer ML, Pokrovskaja K, Grandér D, Xiao G, Rocha S, Lehtiö J, Sangfelt O.
Nature Communications 2012 Jan; 3:976.
Application：WB-Tr, Human, HCT-116 cells.
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Proteomic screen reveals Fbw7 as a modulator of the NF-κB pathway.
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