SMAD4 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of human cell line SCLC-21H.
Western Blot (Cell lysate)
Western Blot analysis of (1) NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), and (2) NBT-II cell lysate (Rat Wistar bladder tumour cells).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human rectum shows strong positivity in glandular cells.
Immunofluorescence
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, cytosol & centrosome. Antibody staining is shown in green.
-
Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human SMAD4.
Immunogen
Recombinant protein corresponding to amino acids 118-240 of human SMAD4.
Sequence
AFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPSSMMVKDEYVHDFEGQPSLSTEGHSIQTIQHPPSNRASTETYSTPALLAPSESNATSTANFPNIPVASTSQPASILGGSHSEGLLQI
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C for short term storage. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western Blot analysis of human cell line SCLC-21H.Western Blot (Cell lysate)
Western Blot analysis of (1) NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), and (2) NBT-II cell lysate (Rat Wistar bladder tumour cells).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human rectum shows strong positivity in glandular cells.Immunofluorescence
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, cytosol & centrosome. Antibody staining is shown in green. -
Gene Info — SMAD4
Entrez GeneID
4089Protein Accession#
Q13485Gene Name
SMAD4
Gene Alias
DPC4, JIP, MADH4
Gene Description
SMAD family member 4
Gene Ontology
HyperlinkGene Summary
mothers against decapentaplegic homolog 4|OTTHUMP00000163548|SMAD
Other Designations
MAD, mothers against decapentaplegic homolog 4|OTTHUMP00000163548|SMAD, mothers against DPP homolog 4|deleted in pancreatic carcinoma locus 4|mothers against decapentaplegic homolog 4|mothers against decapentaplegic, Drosophila, homolog of, 4
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com