THOC1 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human THOC1.
Immunogen
Recombinant protein corresponding to amino acids 154-240 of human THOC1.
Sequence
VFCGRIQLFLARLFPLSEKSGLNLQSQFNLENVTVFNTNEQESTLGQKHTEDREEGMDVEEGEMGDEEAPTTCSIPIDYNLYRKFWS
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:20-1:50)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C for short term storage. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of (1) human cell line RT-4, and (2) human cell line U-251MG sp.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebral cortex shows strong nuclear positivity in neuronal and glial cells.Immunofluorescence
Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles. Antibody staining is shown in green. -
Gene Info — THOC1
Entrez GeneID
9984Protein Accession#
Q96FV9Gene Name
THOC1
Gene Alias
HPR1, P84, P84N5
Gene Description
THO complex 1
Omim ID
606930Gene Ontology
HyperlinkGene Summary
HPR1 is part of the TREX (transcription/export) complex, which includes TEX1 (MIM 606929), THO2 (MIM 300395), ALY (MIM 604171), and UAP56 (MIM 606390).[supplied by OMIM
Other Designations
nuclear matrix protein p84
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Interactome
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Publication Reference
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Differential expression of THOC1 and ALY mRNP biogenesis/export factors in human cancers.
Domínguez-Sanchez MS, Saez C, Japon MA, Aguilera A, Luna R.
BMC Cancer 2011 Feb; 11:77.
Application:IHC-P, Human, Human tissue microarray.
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Differential expression of THOC1 and ALY mRNP biogenesis/export factors in human cancers.
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