GRK6 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot (Cell lysate) analysis of (1) Negative control (vector only transfected HEK293T lysate), and (2) Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.
Immunofluorescence
Immunofluorescent staining of U-2 OS cell line with antibody shows positivity in mitochondria (green).
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human GRK6.
Immunogen
Recombinant protein corresponding to human GRK6.
Sequence
LLFREFCATRPELSRCVAFLDGVAEYEVTPDDKRKACGRQLTQNFLSHTGPDLIPEVPRQLVTNCTQRLEQGPCKDLFQELTRLTHEYLSVAPFADYLDSIYFNRFLQWKWLER
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:20-50)
Western Blot (1:100-250)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot (Cell lysate) analysis of (1) Negative control (vector only transfected HEK293T lysate), and (2) Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.Immunofluorescence
Immunofluorescent staining of U-2 OS cell line with antibody shows positivity in mitochondria (green). -
Gene Info — GRK6
Entrez GeneID
2870Protein Accession#
P43250Gene Name
GRK6
Gene Alias
FLJ32135, GPRK6
Gene Description
G protein-coupled receptor kinase 6
Omim ID
600869Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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