USP25 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human USP25.
Immunogen
Recombinant protein corresponding to human USP25.
Sequence
QPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFK
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-500)
Western Blot (1:100-250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of (1) Human RT-4 cell, (2) Human U-251MG sp cell.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes. -
Gene Info — USP25
Entrez GeneID
29761Protein Accession#
Q9UHP3Gene Name
USP25
Gene Alias
USP21
Gene Description
ubiquitin specific peptidase 25
Omim ID
604736Gene Ontology
HyperlinkGene Summary
Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]).[supplied by OMIM
Other Designations
deubiquitinating enzyme 25|ubiquitin carboxyl-terminal hydrolase 25|ubiquitin specific protease 25|ubiquitin thiolesterase 25|ubiquitin-specific processing protease 25
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Interactome
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Disease
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