BRE polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot (Cell lysate) analysis of (1) Negative control (vector only transfected HEK293T lysate), and (2) Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human kidney shows strong positivity in tubuli.
Immunofluorescence
Immunofluorescent staining of U-2 OS cell line with antibody shows positivity in cytosol (green).
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human BRE.
Immunogen
Recombinant protein corresponding to human BRE.
Sequence
ALNRISPMLSPFISSVVRNGKVGLDATNCLRITDLKSGCTSLTPGPNCDRFKLHIPYAGETLKWDIIFNAQYPELPPDFIFGEDAEFLPDPSALQNLA
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:20-50)
Western Blot (1:100-250)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot (Cell lysate) analysis of (1) Negative control (vector only transfected HEK293T lysate), and (2) Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human kidney shows strong positivity in tubuli.Immunofluorescence
Immunofluorescent staining of U-2 OS cell line with antibody shows positivity in cytosol (green). -
Gene Info — BRE
Entrez GeneID
9577Protein Accession#
Q9NXR7Gene Name
BRE
Gene Alias
BRCC4, BRCC45
Gene Description
brain and reproductive organ-expressed (TNFRSF1A modulator)
Omim ID
610497Gene Ontology
HyperlinkGene Summary
subunit 4|OTTHUMP00000123504|OTTHUMP00000123506|OTTHUMP00000123508
Other Designations
BRCA1/BRCA2-containing complex, subunit 4|OTTHUMP00000123504|OTTHUMP00000123506|OTTHUMP00000123508
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Interactome
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Disease
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