PADI4 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with PADI4 polyclonal antibody (Cat # PAB31520).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human bone marrow with PADI4 polyclonal antibody (Cat # PAB31520) shows strong cytoplasmic and nuclear positivity in subset of hematopoietic cells.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human PADI4.
Immunogen
Recombinant protein corresponding to human PADI4.
Sequence
CEDDEVLDSEDLQDMSLMTLSTKTPKDFFTNHTLVLHVARSEMDKVRVFQATRGKLSSKCSVVLGPKWPSHYLMVPGGKHNMDFYVEALAFPDTDFPGLITLTISLLD
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with PADI4 polyclonal antibody (Cat # PAB31520).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human bone marrow with PADI4 polyclonal antibody (Cat # PAB31520) shows strong cytoplasmic and nuclear positivity in subset of hematopoietic cells. -
Gene Info — PADI4
Entrez GeneID
23569Protein Accession#
Q9UM07Gene Name
PADI4
Gene Alias
PAD, PAD4, PADI5, PDI4, PDI5
Gene Description
peptidyl arginine deiminase, type IV
Gene Ontology
HyperlinkGene Summary
This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response. [provided by RefSeq
Other Designations
HL-60 PAD|OTTHUMP00000002482|PADI-H protein|peptidyl arginine deiminase, type V|protein-arginine deiminase
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Interactome
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Disease
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