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Last updated: 2016/12/4
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LIN28B polyclonal antibody

  • Catalog # : PAB3152
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of LIN28B.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human LIN28B.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Recommend Usage:
  • Western Blot (1:1000)
    Immunohistochemistry (1:10-50)
    Flow cytometry (1:10-50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of LIN28B polyclonal antibody (Cat # PAB3152) in HL-60 cell line lysates (35 ug/lane). LIN28B (arrow) was detected using the purified polyclonal antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human testisreacted with LIN28B polyclonal antibody (Cat # PAB3152), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
    This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HL-60 cells using LIN28B polyclonal antibody (Cat # PAB3152)(bottom histogram) compared to a negative control cell (top histogram).
    FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Protein Accession#:
  • NP_001004317;Q6ZN17
  • Gene Name:
  • LIN28B
  • Gene Alias:
  • CSDD2,FLJ16517
  • Gene Description:
  • lin-28 homolog B (C. elegans)
  • Other Designations:
  • Lin-28.2,OTTHUMP00000016909,lin-28 homolog B
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