LIN28B polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of LIN28B.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human LIN28B.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of LIN28B polyclonal antibody (Cat # PAB3152) in HL-60 cell line lysates (35 ug/lane). LIN28B (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human testisreacted with LIN28B polyclonal antibody (Cat # PAB3152), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Flow Cytometry
Flow cytometric analysis of HL-60 cells using LIN28B polyclonal antibody (Cat # PAB3152)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — LIN28B
Entrez GeneID
389421Protein Accession#
NP_001004317;Q6ZN17Gene Name
LIN28B
Gene Alias
CSDD2, FLJ16517
Gene Description
lin-28 homolog B (C. elegans)
Omim ID
611044Gene Ontology
HyperlinkOther Designations
Lin-28.2|OTTHUMP00000016909|lin-28 homolog B
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Interactome
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Disease
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Publication Reference
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Identification and characterization of lin-28 homolog B (LIN28B) in human hepatocellular carcinoma.
Guo Y, Chen Y, Ito H, Watanabe A, Ge X, Kodama T, Aburatani H.
Gene 2006 Dec; 384:51.
Application:IF, WB-Ce, WB-Tr, Human, Monkey, COS-7, Hep-3B, Hep-G2, HLE, Huh7, MCF-7 cells, human hepatocellular carcinoma tissues.
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Identification and characterization of lin-28 homolog B (LIN28B) in human hepatocellular carcinoma.
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