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Last updated: 2016/12/4
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DDX3X polyclonal antibody

  • Catalog # : PAB3151
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of DDX3X.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human DDX3X.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Recommend Usage:
  • Western Blot (1:1000)
    Immunohistochemistry (1:10-50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of DDX3X polyclonal antibody (Cat # PAB3151) in HeLa cell line lysates (35 ug/lane). DDX3X (arrow) was detected using the purified polyclonal antibody.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of DDX3X (arrow) using rabbit DDX3X polyclonal antibody (Cat # PAB3151). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DDX3 gene (Lane 2) (Origene Technologies).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with DDX3X polyclonal antibody (Cat # PAB3151) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 1654
  • Protein Accession#:
  • NP_001347;O00571
  • Gene Name:
  • DDX3X
  • Gene Alias:
  • DBX,DDX14,DDX3,HLP2
  • Gene Description:
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
  • Gene Summary:
  • DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which interacts specifically with hepatitis C virus core protein resulting a change in intracellular location. This gene has a homolog located in the nonrecombining region of the Y chromosome. The protein sequence is 91% identical between this gene and the Y-linked homolog. [provided by RefSeq
  • Other Designations:
  • ATP-dependent RNA helicase DDX3X,CAP-Rf,DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3,DEAD/H box-3,helicase like protein 2
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