CD9 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of CD9 polyclonal antibody (Cat # PAB3149) in HepG2 cell line lysates (35 ug/lane). CD9 (arrow) was detected using the purified polyclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of CD9 (arrow) using rabbit CD9 polyclonal antibody (Cat # PAB3149). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD9 gene (Lane 2) (Origene Technologies).
Immunofluorescence
Immunofluorescence analysis of CD9 polyclonal antibody (Cat # PAB3149) in HeLa cells. Primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Flow Cytometry
Flow cytometric analysis of Jurkat cells using CD9 polyclonal antibody (Cat # PAB3149)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CD9.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human CD9.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunofluorescence (1:10-50)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CD9 polyclonal antibody (Cat # PAB3149) in HepG2 cell line lysates (35 ug/lane). CD9 (arrow) was detected using the purified polyclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of CD9 (arrow) using rabbit CD9 polyclonal antibody (Cat # PAB3149). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD9 gene (Lane 2) (Origene Technologies).Immunofluorescence
Immunofluorescence analysis of CD9 polyclonal antibody (Cat # PAB3149) in HeLa cells. Primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.Flow Cytometry
Flow cytometric analysis of Jurkat cells using CD9 polyclonal antibody (Cat # PAB3149)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — CD9
Entrez GeneID
928Protein Accession#
NP_001760;P21926Gene Name
CD9
Gene Alias
5H9, BA2, BTCC-1, DRAP-27, GIG2, MIC3, MRP-1, P24, TSPAN29
Gene Description
CD9 molecule
Omim ID
143030Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. In addition, the protein appears to promote muscle cell fusion and support myotube maintenance. [provided by RefSeq
Other Designations
5H9 antigen|CD9 antigen|CD9 antigen (p24)|OTTHUMP00000041574|OTTHUMP00000041576|antigen defined by monoclonal antibody 602-29|growth-inhibiting gene 2 protein|leukocyte antigen MIC3|motility related protein|motility related protein-1|p24 antigen
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Interactome
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Pathway
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Disease
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Publication Reference
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The tetraspanin CD9 inhibits the proliferation and tumorigenicity of human colon carcinoma cells.
Ovalle S, Gutierrez-Lopez MD, Olmo N, Turnay J, Lizarbe MA, Majano P, Molina-Jimenez F, Lopez-Cabrera M, Yanez-Mo M, Sanchez-Madrid F, Cabanas C.
International Journal of Cancer 2007 Nov; 121(10):2140.
Application：Flow Cyt, Func, WB-Ti, Human, Mouse, BCS-TC2.2, Colo320, Colo320-CD9, HT29 cells, Mouse tumors.
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The transferrin receptor and the tetraspanin web molecules CD9, CD81, and CD9P-1 are differentially sorted into exosomes after TPA treatment of K562 cells.
Abache T, Le Naour F, Planchon S, Harper F, Boucheix C, Rubinstein E.
Journal of Cellular Biochemistry 2007 Oct; 102(3):650.
Application：Flow Cyt, IF, IP, WB, Human, K-562 cells.
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A novel cysteine cross-linking method reveals a direct association between claudin-1 and tetraspanin CD9.
Kovalenko OV, Yang XH, Hemler ME.
Molecular & Cellular Proteomics 2007 Nov; 6(11):1855.
Application：IF, IP, WB, Human, A431, A-549, C2BBe1, MCF-7, MDCK cells.
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The tetraspanin CD9 inhibits the proliferation and tumorigenicity of human colon carcinoma cells.
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