ABCF1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of (1) human cell line RT-4, and (2) human cell line U-251MG sp.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human small intestine shows strong cytoplasmic positivity in glandular cells.
Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human ABCF1.
Immunogen
Recombinant protein corresponding to amino acids 85-196 of human ABCF1.
Sequence
KKDVDDDGEEKELMERLKKLSVPTSDEEDEVPAPKPRGGKKTKGGNVFAALIQDQSEEEEEEEKHPPKPAKPEKNRINKAVSEEQQPALKGKKGKEEKSKGKAKPQNKFAAL
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C for short term storage. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of (1) human cell line RT-4, and (2) human cell line U-251MG sp.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human small intestine shows strong cytoplasmic positivity in glandular cells.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green. -
Gene Info — ABCF1
Entrez GeneID
23Protein Accession#
Q8NE71Gene Name
ABCF1
Gene Alias
ABC27, ABC50
Gene Description
ATP-binding cassette, sub-family F (GCN20), member 1
Omim ID
603429Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the GCN20 subfamily. Unlike other members of the superfamily, this protein lacks the transmembrane domains which are characteristic of most ABC transporters. This protein may be regulated by tumor necrosis factor-alpha and play a role in enhancement of protein synthesis and the inflammation process. [provided by RefSeq
Other Designations
ATP-binding cassette 50 (TNF-alpha stimulated)|ATP-binding cassette, sub-family F, member 1|OTTHUMP00000029110|OTTHUMP00000029111|TNFalpha-inducible ATP-binding protein
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Interactome
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Disease
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