JMJD1B polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of (1) human cell line RT-4, and (2) human cell line U-251MG sp.
Western Blot
Western Blot analysis of (1) NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), and (2) NBT-II cell lysate (Rat Wistar bladder tumour cells).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human fallopian tube shows nuclear positivity in glandular cells.
Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human JMJD1B.
Immunogen
Recombinant protein corresponding to amino acids 307-448 of human JMJD1B.
Sequence
GEVDSNGSDGGEASRGPWKGGNASGEPGLDQRAKQPPSTFVPQINRNIRFATYTKENGRTLVVQDEPVGGDTPASFTPYSTATGQTPLAPEVGGAENKEAGKTLEQVGQGIVASAAVVTTASSTPNTVRISDTGLAAGTVPE
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C for short term storage. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of (1) human cell line RT-4, and (2) human cell line U-251MG sp.Western Blot
Western Blot analysis of (1) NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), and (2) NBT-II cell lysate (Rat Wistar bladder tumour cells).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human fallopian tube shows nuclear positivity in glandular cells.Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green. -
Gene Info — JMJD1B
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Interactome
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Disease
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Publication Reference
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CCAT1 is an enhancer-templated RNA that predicts BET sensitivity in colorectal cancer.
McCleland ML, Mesh K, Lorenzana E, Chopra VS, Segal E, Watanabe C, Haley B, Mayba O, Yaylaoglu M, Gnad F, Firestein R.
The Journal of Clinical Investigation 2016 Feb; 126(2):639.
Application：IF, WB-Tr, Human, HCT-116, RKO cells.
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CCAT1 is an enhancer-templated RNA that predicts BET sensitivity in colorectal cancer.
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