LGI2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human LGI2.
Immunogen
Recombinant protein corresponding to human LGI2.
Sequence
DLTHLSLANNHIKALPRDVFSDLDSLIELDLRGNKFECDCKAKWLYLWLKMTNSTVSDVLCIGPPEYQEKKLNDVTSFDYECTTTDFVVHQTLPYQSVSVDTFNSKNDVYVAIAQPSMENCMVLEW
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:20-1:50)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with LGI2 polyclonal antibody (Cat # PAB31471).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human parathyroid gland with LGI2 polyclonal antibody (Cat # PAB31471) shows moderate cytoplasmic positivity in glandular cells.Immunofluorescence
Immunofluorescent staining of U-251 MG cells with LGI2 polyclonal antibody (Cat # PAB31471) (Green) shows localization to centrosome. -
Gene Info — LGI2
Entrez GeneID
55203Protein Accession#
Q8N0V4Gene Name
LGI2
Gene Alias
FLJ10675, KIAA1916, LGIL2, MGC126808, MGC126810
Gene Description
leucine-rich repeat LGI family, member 2
Omim ID
608301Gene Ontology
HyperlinkGene Summary
O
Other Designations
LGI1-like protein 2|OTTHUMP00000123374|leucine-rich, glioma inactivated 2
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