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Last updated: 2017/5/28
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CD9 polyclonal antibody

  • Catalog # : PAB3146
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of CD9.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human CD9.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Recommend Usage:
  • Western Blot (1:1000)
    Flow cytometry (1:10-50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of CD9 polyclonal antibody (Cat # PAB3146) in Jurkat cell line lysates (35 ug/lane). CD9 (arrow) was detected using the purified polyclonal antibody.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of CD9 (arrow) using rabbit CD9 polyclonal antibody (Cat # PAB3146). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD9 gene (Lane 2) (Origene Technologies).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of Jurkat cells using CD9 polyclonal antibody (Cat # PAB3146)(bottom histogram) compared to a negative control cell (top histogram).
    FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 928
  • Protein Accession#:
  • NP_001760;P21926
  • Gene Name:
  • CD9
  • Gene Alias:
  • 5H9,BA2,BTCC-1,DRAP-27,GIG2,MIC3,MRP-1,P24,TSPAN29
  • Gene Description:
  • CD9 molecule
  • Gene Summary:
  • The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. In addition, the protein appears to promote muscle cell fusion and support myotube maintenance. [provided by RefSeq
  • Other Designations:
  • 5H9 antigen,CD9 antigen,CD9 antigen (p24),OTTHUMP00000041574,OTTHUMP00000041576,antigen defined by monoclonal antibody 602-29,growth-inhibiting gene 2 protein,leukocyte antigen MIC3,motility related protein,motility related protein-1,p24 antigen
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