SEMA4D polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human SEMA4D.
Immunogen
Recombinant protein corresponding to human SEMA4D.
Sequence
LLIGKKKPKSDFCDREQSLKETLVEPGSFSQQNGEHPKPALDTGYETEQDTITSKVPTDREDSQRIDDLSARDKPFDVKCELKFADSDADGD
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of HDLM-2 cells with SEMA4D polyclonal antibody (Cat # PAB31457).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lymph node with SEMA4D polyclonal antibody (Cat # PAB31457) shows cytoplasmic positivity in germinal center cells and non-germinal center cells. -
Gene Info — SEMA4D
Entrez GeneID
10507Protein Accession#
Q92854Gene Name
SEMA4D
Gene Alias
C9orf164, CD100, FLJ33485, FLJ34282, FLJ39737, FLJ46484, M-sema-G, MGC169138, MGC169141, SEMAJ, coll-4
Gene Description
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
Omim ID
601866Gene Ontology
HyperlinkGene Summary
sema domain
Other Designations
OTTHUMP00000021622|OTTHUMP00000070035|sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D|semaphorin 4D
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Interactome
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Pathway
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Disease
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Publication Reference
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The combined expression of Semaphorin4D and PlexinB1 predicts disease recurrence in colorectal cancer.
Ikeya T, Maeda K, Nagahara H, Shibutani M, Iseki Y, Hirakawa K.
BMC Cancer 2016 Jul; 16:525.
Application:IHC-P, Human, Human colorectal cancer specimens.
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The combined expression of Semaphorin4D and PlexinB1 predicts disease recurrence in colorectal cancer.
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