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GAP43 polyclonal antibody

  • Catalog # : PAB31439
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial recombinant human GAP43.
  • Immunogen:
  • Recombinant protein corresponding to human GAP43.
  • Sequence:
  • KEEPKQADVPAAVTAAAATTPAAEDAAAKATAQPPTETGESSQAEENIEAVDETKPKESARQDE
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunofluorescence (1-4 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:1000-1:2500)
    Western Blot (1:100-1:250)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with GAP43 polyclonal antibody (Cat # PAB31439).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebral cortex with GAP43 polyclonal antibody (Cat # PAB31439) shows distinct neuropil positivity.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of SH-SY5Y cells with GAP43 polyclonal antibody (Cat # PAB31439) (Green) shows localization to plasma membrane.
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 2596
  • Gene Name:
  • GAP43
  • Gene Alias:
  • B-50,PP46
  • Gene Description:
  • growth associated protein 43
  • Gene Summary:
  • The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • axonal membrane protein GAP-43,calmodulin-binding protein P-57,nerve growth-related peptide GAP43,neural phosphoprotein B-50,neuromodulin,neuron growth-associated protein 43,protein F1
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