NLRP3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human NLRP3.
Immunogen
Recombinant protein corresponding to human NLRP3.
Sequence
FKMHLEDYPPQKGCIPLPRGQTEKADHVDLATLMIDFNGEEKAWAMAVWIFAAINRRDLYEKAKRDEPKWGSDNARVSNPTVICQEDSIEEEWMGLLEYLSRISICKMKKDYRKKYR
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with NLRP3 polyclonal antibody (Cat # PAB31435) shows moderate cytoplasmic positivity in germinal and non-germinal center cells. -
Gene Info — NLRP3
Entrez GeneID
114548Protein Accession#
Q96P20Gene Name
NLRP3
Gene Alias
AGTAVPRL, AII, AII/AVP, AVP, C1orf7, CIAS1, CLR1.1, FCAS, FCU, FLJ95925, MWS, NALP3, PYPAF1
Gene Description
NLR family, pyrin domain containing 3
Gene Ontology
HyperlinkGene Summary
This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq
Other Designations
AII/AVP receptor-like|NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3|NACHT, LRR and PYD containing protein 3|OTTHUMP00000038408|OTTHUMP00000038409|OTTHUMP00000038410|PYRIN-containing APAF1-like protein 1|angiotensin/vasopressin receptor
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Interactome
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Disease
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Publication Reference
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NLRP3 Inflammasome Expression and Activation in Human Atherosclerosis.
Paramel Varghese G, Folkersen L, Strawbridge RJ, Halvorsen B, Yndestad A, Ranheim T, Krohg‐Sørensen K, Skjelland M, Espevik T, Aukrust P, Lengquist M, Hedin U, Jansson J, Fransén K, Hansson GK, Eriksson P, Sirsjö A.
Journal of the American Heart Association 2016 May; 5(5):e003031.
Application:IHC-P, Human, Human carotid artery plaque tissues.
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Unsaturated fatty acids prevent activation of NLRP3 inflammasome in human monocytes/macrophages.
L'homme L, Esser N, Riva L, Scheen A, Paquot N, Piette J, Legrand-Poels S.
Journal of Lipid Research 2013 Nov; 54(11):2998.
Application:WB-Ce, WB-Tr, Human, Human monocytes/macrophages, THP-1 cells.
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Cutting edge: miR-223 and EBV miR-BART15 regulate the NLRP3 inflammasome and IL-1β production.
Haneklaus M, Gerlic M, Kurowska-Stolarska M, Rainey AA, Pich D, McInnes IB, Hammerschmidt W, O'Neill LA, Masters SL.
Journal of Immunology 2012 Oct; 189(8):3795.
Application:WB-Ce, WB-Tr, Human, Human macrophages, THP-1 cells.
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Chlamydial infection of monocytes stimulates IL-1beta secretion through activation of the NLRP3 inflammasome.
Abdul-Sater AA, Saïd-Sadier N, Padilla EV, Ojcius DM.
Microbes and Infection 2010 Aug; 12(8-9):652.
Application:WB-Tr, Human, THP-1 cells.
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Aspergillus fumigatus stimulates the NLRP3 inflammasome through a pathway requiring ROS production and the Syk tyrosine kinase.
Saïd-Sadier N, Padilla E, Langsley G, Ojcius DM.
PLoS One 2010 Apr; 5(4):e10008.
Application:WB-Tr, Human, THP-1 cells.
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Inflammasome-dependent caspase-1 activation in cervical epithelial cells stimulates growth of the intracellular pathogen Chlamydia trachomatis.
Abdul-Sater AA, Koo E, Hacker G, Ojcius DM.
The Journal of Biological Chemistry 2009 Sep; 284(39):26789.
Application:WB-Tr, Human, HeLa cells.
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NLRP3 Inflammasome Expression and Activation in Human Atherosclerosis.
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