ARMET polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of HepG2 cell lysate with ARMET polyclonal antibody (Cat # PAB31425).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human pancreas with ARMET polyclonal antibody (Cat # PAB31425) shows strong cytoplasmic positivity in exocrine glandular cells.
Immunofluorescence
Immunofluorescent staining of U-2 OS cells with ARMET polyclonal antibody (Cat # PAB31425) (Green) shows localization to endoplasmic reticulum.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human ARMET.
Immunogen
Recombinant protein corresponding to human ARMET.
Sequence
CEVCISYLGRFYQDLKDRDVTFSPATIENELIKFCREAR
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of HepG2 cell lysate with ARMET polyclonal antibody (Cat # PAB31425).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human pancreas with ARMET polyclonal antibody (Cat # PAB31425) shows strong cytoplasmic positivity in exocrine glandular cells.Immunofluorescence
Immunofluorescent staining of U-2 OS cells with ARMET polyclonal antibody (Cat # PAB31425) (Green) shows localization to endoplasmic reticulum. -
Gene Info — ARMET
Entrez GeneID
7873Protein Accession#
P55145Gene Name
ARMET
Gene Alias
ARP, MANF, MGC142148, MGC142150
Gene Description
arginine-rich, mutated in early stage tumors
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and promotes cell proliferation. The protein was initially thought to be longer at the N-terminus and to contain an arginine-rich region but transcribed evidence indicates a smaller open reading frame that does not encode the arginine tract. The presence of a specific mutation changing the previously numbered codon 50 from ATG to AGG, or deletion of that codon, has been reported in a variety of solid tumors. With the protein size correction, this codon is now identified as the initiation codon. [provided by RefSeq
Other Designations
arginine-rich protein
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Interactome
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Publication Reference
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Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Charlotte Stadler, Elton Rexhepaj, Vasanth R Singan, Robert F Murphy, Rainer Pepperkok, Mathias Uhlén, Jeremy C Simpson, Emma Lundberg.
Nature Methods 2013 Apr; 10(4):315.
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Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
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