MCAM polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of WM-115 cell lysate with MCAM polyclonal antibody (Cat # PAB31408).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon with MCAM polyclonal antibody (Cat # PAB31408) shows strong positivity in endothelial cells.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human MCAM.
Immunogen
Recombinant protein corresponding to human MCAM.
Sequence
LNYRLPSGNHMKESREVTVPVFYPTEKVWLEVEPVGMLKEGDRVEIRCLADGNPPPHFSISKQNPSTREAEEETTNDNGVLVLEPARKEHSGRYECQGLDLDTMISLLSEPQELLVNYVSDVRVSPAAPERQEGSSLTLTCEAESSQDLE
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of WM-115 cell lysate with MCAM polyclonal antibody (Cat # PAB31408).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon with MCAM polyclonal antibody (Cat # PAB31408) shows strong positivity in endothelial cells. -
Gene Info — MCAM
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Interactome
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Publication Reference
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Glutamate dependent NMDA receptor 2D is a novel angiogenic tumour endothelial marker in colorectal cancer.
Ferguson HJ, Wragg JW, Ward S, Heath VL, Ismail T, Bicknell R.
Oncotarget 2016 Apr; 7(15):20440.
Application：WB-Ce, Human, HUVECs.
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Glutamate dependent NMDA receptor 2D is a novel angiogenic tumour endothelial marker in colorectal cancer.
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