RPN2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: RT-4 and Lane 2: U-251 MG sp cell lysates with RPN2 polyclonal antibody (Cat # PAB31400).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human epididymis with RPN2 polyclonal antibody (Cat # PAB31400) shows strong cytoplasmic positivity in glandular cells.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human RPN2.
Immunogen
Recombinant protein corresponding to human RPN2.
Sequence
SISNETKDLLLAAVSEDSSVTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSIVEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTE
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: RT-4 and Lane 2: U-251 MG sp cell lysates with RPN2 polyclonal antibody (Cat # PAB31400).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human epididymis with RPN2 polyclonal antibody (Cat # PAB31400) shows strong cytoplasmic positivity in glandular cells. -
Gene Info — RPN2
Entrez GeneID
6185Protein Accession#
P04844Gene Name
RPN2
Gene Alias
RIBIIR, RPN-II, RPNII, SWP1
Gene Description
ribophorin II
Omim ID
180490Gene Ontology
HyperlinkGene Summary
This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit
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Interactome
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Pathway
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Disease
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Publication Reference
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Candidate serological biomarkers for cancer identified from the secretomes of 23 cancer cell lines and the human protein atlas.
Wu CC, Hsu CW, Chen CD, Yu CJ, Chang KP, Tai DI, Liu HP, Su WH, Chang YS, Yu JS.
Molecular & Cellular Proteomics 2010 Jun; 9(6):1100.
Application：IHC, Human, Colon, Liver, Bladder, Breast, Lung cancer.
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Candidate serological biomarkers for cancer identified from the secretomes of 23 cancer cell lines and the human protein atlas.
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