CDH1 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CDH1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human CDH1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CDH1 polyclonal antibody (Cat # PAB3139) in 293 cell line lysates (35 ug/lane). CDH1 (arrow) was detected using the purified polyclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of CDH1 (arrow) using rabbit CDH1 polyclonal antibody (Cat # PAB3139). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH1 gene (Lane 2) (Origene Technologies).Flow Cytometry
Flow cytometric analysis of NCI-H292 cells using CDH1 polyclonal antibody (Cat # PAB3139)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — CDH1
Entrez GeneID
999Protein Accession#
NP_004351;P12830Gene Name
CDH1
Gene Alias
Arc-1, CD324, CDHE, ECAD, LCAM, UVO
Gene Description
cadherin 1, type 1, E-cadherin (epithelial)
Gene Ontology
HyperlinkGene Summary
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq
Other Designations
cadherin 1, E-cadherin (epithelial)|cadherin 1, type 1|calcium-dependent adhesion protein, epithelial|cell-CAM 120/80|uvomorulin
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Interactome
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Pathway
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Disease
- Adenocarcinoma
- Asthma
- Breast cancer
- Breast Neoplasms
- Bronchiolitis
- Carcinoma
- Cleft Lip
- Cleft Palate
- Colitis
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Publication Reference
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KCl cotransporter-3 down-regulates E-cadherin/beta-catenin complex to promote epithelial-mesenchymal transition.
Hsu YM, Chen YF, Chou CY, Tang MJ, Chen JH, Wilkins RJ, Ellory JC, Shen MR.
Cancer Research 2007 Nov; 67(22):11064.
Application:IF, IP, WB, Human, CaSki, SiHa cells, Human cervical cancer.
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Plakoglobin, or an 83-kD homologue distinct from beta-catenin, interacts with E-cadherin and N-cadherin.
Knudsen KA, Wheelock MJ.
The Journal of Cell Biology 1993 Sep; 118(4):672.
Application:IF, IP, WB-Ce, Chicken, Human, Chicken cardiac myocytes, Human epithelial cells.
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Characterization and chromosomal localization of the gene encoding the human cell adhesion molecule uvomorulin.
Mansouri A, Spurr N, Goodfellow PN, Kemler R.
Differentiation 1988 Jun; 38(1):67.
Application:IHC, IP, Mouse, Mouse preimplantation embryos.
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KCl cotransporter-3 down-regulates E-cadherin/beta-catenin complex to promote epithelial-mesenchymal transition.
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