ALDH5A1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ALDH5A1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human ALDH5A1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of ALDH5A1 (arrow) using rabbit ALDH5A1 polyclonal antibody (Cat # PAB3134). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALDH5A1 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with ALDH5A1 polyclonal antibody (Cat # PAB3134) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Flow Cytometry
Flow cytometric analysis of ATDC5 cells using ALDH5A1 polyclonal antibody (Cat # PAB3134)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — ALDH5A1
Entrez GeneID
7915Protein Accession#
NP_001071;P51649Gene Name
ALDH5A1
Gene Alias
SSADH, SSDH
Gene Description
aldehyde dehydrogenase 5 family, member A1
Gene Ontology
HyperlinkGene Summary
This protein belongs to the aldehyde dehydrogenase family of proteins. This gene encodes a mitochondrial NAD(+)-dependent succinic semialdehyde dehydrogenase. A deficiency of this enzyme, known as 4-hydroxybutyricaciduria, is a rare inborn error in the metabolism of the neurotransmitter 4-aminobutyric acid (GABA). In response to the defect, physiologic fluids from patients accumulate GHB, a compound with numerous neuromodulatory properties. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
Other Designations
NAD(+)-dependent succinic semialdehyde dehydrogenase|OTTHUMP00000016088|aldehyde dehydrogenase 5A1|mitochondrial succinate semialdehyde dehydrogenase|succinate-semialdehyde dehydrogenase
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Interactome
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Pathway
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Disease
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Publication Reference
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Therapeutic concepts in succinate semialdehyde dehydrogenase (SSADH; ALDH5a1) deficiency (gamma-hydroxybutyric aciduria). Hypotheses evolved from 25 years of patient evaluation, studies in Aldh5a1-/- mice and characterization of gamma-hydroxybutyric acid pharmacology.
Knerr I, Pearl PL, Bottiglieri T, Snead OC, Jakobs C, Gibson KM.
Journal of Inherited Metabolic Disease 2007 Jun; 30(3):279.
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SSADH variation in primates: intra- and interspecific data on a gene with a potential role in human cognitive functions.
Blasi P, Palmerio F, Aiello A, Rocchi M, Malaspina P, Novelletto A.
Journal of Molecular Evolution 2006 Jul; 63(1):54.
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A functional polymorphism in the succinate-semialdehyde dehydrogenase (aldehyde dehydrogenase 5 family, member A1) gene is associated with cognitive ability.
Plomin R, Turic DM, Hill L, Turic DE, Stephens M, Williams J, Owen MJ, O'Donovan MC.
Molecular Psychiatry 2004 Jun; 9(6):582.
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Therapeutic concepts in succinate semialdehyde dehydrogenase (SSADH; ALDH5a1) deficiency (gamma-hydroxybutyric aciduria). Hypotheses evolved from 25 years of patient evaluation, studies in Aldh5a1-/- mice and characterization of gamma-hydroxybutyric acid pharmacology.
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