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Last updated: 2017/5/21
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ALDH5A1 polyclonal antibody

  • Catalog # : PAB3134
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of ALDH5A1.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human ALDH5A1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Recommend Usage:
  • Western Blot (1:1000)
    Immunohistochemistry (1:10-50)
    Flow cytometry (1:10-50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of ALDH5A1 (arrow) using rabbit ALDH5A1 polyclonal antibody (Cat # PAB3134). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALDH5A1 gene (Lane 2) (Origene Technologies).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with ALDH5A1 polyclonal antibody (Cat # PAB3134) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of ATDC5 cells using ALDH5A1 polyclonal antibody (Cat # PAB3134)(bottom histogram) compared to a negative control cell (top histogram).
    FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 7915
  • Protein Accession#:
  • NP_001071;P51649
  • Gene Name:
  • ALDH5A1
  • Gene Alias:
  • SSADH,SSDH
  • Gene Description:
  • aldehyde dehydrogenase 5 family, member A1
  • Gene Summary:
  • This protein belongs to the aldehyde dehydrogenase family of proteins. This gene encodes a mitochondrial NAD(+)-dependent succinic semialdehyde dehydrogenase. A deficiency of this enzyme, known as 4-hydroxybutyricaciduria, is a rare inborn error in the metabolism of the neurotransmitter 4-aminobutyric acid (GABA). In response to the defect, physiologic fluids from patients accumulate GHB, a compound with numerous neuromodulatory properties. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
  • Other Designations:
  • NAD(+)-dependent succinic semialdehyde dehydrogenase,OTTHUMP00000016088,aldehyde dehydrogenase 5A1,mitochondrial succinate semialdehyde dehydrogenase,succinate-semialdehyde dehydrogenase
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