PAPOLA polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of 40 ug nuclear extracts of Hela cells.
Enzyme-linked Immunoabsorbent Assay
ELISA is a quantitative method used to determine the titer of the antibody using a serial dilution of antibody against PAPOLA. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:22500.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PAPOLA.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PAPOLA.
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
ELISA (1:100-500)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In whole antiserum (0.05% sodium azide).
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of 40 ug nuclear extracts of Hela cells.Enzyme-linked Immunoabsorbent Assay
ELISA is a quantitative method used to determine the titer of the antibody using a serial dilution of antibody against PAPOLA. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:22500. -
Gene Info — PAPOLA
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Interactome
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Pathway
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