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Histone H3 (K36me3) polyclonal antibody

  • Catalog # : PAB31321
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Histone H3 (K36me3).
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to Histone H3, trimethylated at lysine 36.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:100-500)
    Western Blot (1:1000)
    ChIP (5-10 ul/CHIP)
    Dot Blot (1:100000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In whole antiserum (0.05% sodium azide).
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • ChIP
  • ChIP
  • ChIP was performed on sheared chromatin from 1 million HeLaS3 cells. IgG (2 ug/IP) was used as a negative IP control. The IP'd DNA was analysed by QPCR with optimized PCR primer pairs for the coding and promoter region of the active GAPDH gene, for the coding region of the inactive TSH2B gene and for the Sat2 satellite repeat.
  • ChIP
  • ChIP
  • ChIP assays were performed using human osteosarcoma (U2OS) cells. A titration of the antibody consisting of 2, 5, 10 and 15 ul per ChIP experiment was analysed. IgG (5 ug/IP) was used as a negative IP control. Quantitative PCR was performed using primer sets for the housekeeping gene GAPDH and for myogenic differentiation gene (MYOD). The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis.
  • ChIP-Seq
  • ChIP-Seq
  • The figure shows the results in 200 kb regions of chromosome 12 (including the GAPDH positive control), 6 and 7 and 14. These results clearly show an enrichment of the H3K36me3 at active genes.
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate) analysis of 15 ug histone extracts of HeLa cells.
  • ELISA
  • ELISA
  • ELISA is a quantitative method used to determine the titer of the antibody using a serial dilution of antibody against Histone H3 (K36me3). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:12700.
  • Dot Blot
  • Dot Blot
  • Cross reactivity test using the Histone H3 (K36me3) antibody.
    Dot Blot analysis was performed with peptides containing containing other modifications of histone H3.Other histone modifications include monomethylation and dimethylation of the same lysine and monomethylation, dimethylation and trimethylation of lysine 9, 27 and 79. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:100000. The figure shows a high specificity of the antibody for the modification of interest.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 8350
  • Gene Name:
  • HIST1H3A
  • Gene Alias:
  • H3/A,H3FA
  • Gene Description:
  • histone cluster 1, H3a
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
  • Other Designations:
  • H3 histone family, member A,histone 1, H3a
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