CDH7 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of CDH7 polyclonal antibody (Cat # PAB3132) in K-562 cell line lysates (35 ug/lane). CDH7 (arrow) was detected using the purified polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human prostate carcinomareacted with CDH7 polyclonal antibody (Cat # PAB3132), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow Cytometry
Flow cytometric analysis of NCI-H292 cells using CDH7 polyclonal antibody (Cat # PAB3132)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CDH7.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human CDH7.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CDH7 polyclonal antibody (Cat # PAB3132) in K-562 cell line lysates (35 ug/lane). CDH7 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human prostate carcinomareacted with CDH7 polyclonal antibody (Cat # PAB3132), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Flow Cytometry
Flow cytometric analysis of NCI-H292 cells using CDH7 polyclonal antibody (Cat # PAB3132)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — CDH7
Entrez GeneID
1005Protein Accession#
NP_387450;Q9ULB5Gene Name
CDH7
Gene Alias
CDH7L1
Gene Description
cadherin 7, type 2
Omim ID
605806Gene Ontology
HyperlinkGene Summary
This gene is a type II classical cadherin from the cadherin superfamily. The encoded membrane protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Cadherins mediate cell-cell binding in a homophilic manner, contributing to the sorting of heterogeneous cell types and the maintenance of orderly structures. Alternative splicing in the 5' UTR of this gene yields variant transcripts encoding the same protein. [provided by RefSeq
Other Designations
OTTHUMP00000163685|cadherin-7
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Interactome
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Disease
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Publication Reference
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Involvement of cadherins 7 and 20 in mouse embryogenesis and melanocyte transformation.
Moore R, Champeval D, Denat L, Tan SS, Faure F, Julien-Grille S, Larue L.
Oncogene 2004 Sep; 23(40):6726.
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Characterization of three novel human cadherin genes (CDH7, CDH19, and CDH20) clustered on chromosome 18q22-q23 and with high homology to chicken cadherin-7.
Kools P, Van Imschoot G, van Roy F.
Genomics 2000 Sep; 68(3):283.
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Identification of three human type-II classic cadherins and frequent heterophilic interactions between different subclasses of type-II classic cadherins.
Shimoyama Y, Tsujimoto G, Kitajima M, Natori M.
The Biochemical Journal 2000 Jul; 349(Pt 1):159.
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Involvement of cadherins 7 and 20 in mouse embryogenesis and melanocyte transformation.
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