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Last updated: 2018/1/21

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Flp polyclonal antibody

  • Catalog # : PAB31306
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of FIp.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to N-terminus of Flp recombinase.
  • Host:
  • Rabbit
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:50)
    Western Blot (1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In whole antiserum (0.05% sodium azide).
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Western Blot analysis of (1) whole cell extracts of untransfected 293 cells, (2) whole cell extracts of 293 cells transfected with Cre, (3) whole cell extracts of 293 cells transfected with Dre, (4) whole cell extracts of 293 cells transfected with Flp.
  • ELISA
  • ELISA
  • ELISA is a quantitative method used to determine the titer of the antibody using a serial dilution of antibody against Flp in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2000.
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