RUNX1T1 polyclonal antibody
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More Files
- More Functions
ChIP
ChIP assays were performed using SKNO-1 cells. Sheared chromatin from 1.25 million cells and 4 ul of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2, OGG1 and VEGF genes. The figure shows the occupancy, calculated as the ratio + control/background for which the H2B gene was used.
ChIP-Seq
The figure shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.
Enzyme-linked Immunoabsorbent Assay
ELISA is a quantitative method used to determine the titer of the antibody using a serial dilution of antibody against human RUNX1T1. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:1300.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RUNX1T1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus and the central region of human RUNX1T1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
ELISA (1:100)
ChIP (4 ug/ChIP)
The optimal working dilution should be determined by the end user.Storage Buffer
In whole antiserum (0.05% sodium azide).
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
ChIP assays were performed using SKNO-1 cells. Sheared chromatin from 1.25 million cells and 4 ul of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2, OGG1 and VEGF genes. The figure shows the occupancy, calculated as the ratio + control/background for which the H2B gene was used.ChIP-Seq
The figure shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.Enzyme-linked Immunoabsorbent Assay
ELISA is a quantitative method used to determine the titer of the antibody using a serial dilution of antibody against human RUNX1T1. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:1300. -
Gene Info — RUNX1T1
Entrez GeneID
862Protein Accession#
Q06455Gene Name
RUNX1T1
Gene Alias
AML1T1, CBFA2T1, CDR, ETO, MGC2796, MTG8, MTG8b, ZMYND2
Gene Description
runt-related transcription factor 1; translocated to, 1 (cyclin D-related)
Omim ID
133435Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Several transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq
Other Designations
acute myelogenous leukemia 1 translocation 1 protein|acute myelogenous leukemia 1 translocation 1, cyclin-D related|core-binding factor, runt domain, alpha subunit 2; translocated to, 1; cyclin D-related|eight twenty one protein|myeloid translocation gene
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Interactome
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Pathway
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Publication Reference
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ERG and FLI1 binding sites demarcate targets for aberrant epigenetic regulation by AML1-ETO in acute myeloid leukemia.
Martens JH, Mandoli A, Simmer F, Wierenga BJ, Saeed S, Singh AA, Altucci L, Vellenga E, Stunnenberg HG.
Blood 2012 Nov; 120(19):4038.
Application：ChIP-Seq, Human, SKNO-1 cells.
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ERG and FLI1 binding sites demarcate targets for aberrant epigenetic regulation by AML1-ETO in acute myeloid leukemia.
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