Dre polyclonal antibody
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More Files
- More Functions
Western Blot
Western Blot analysis of (1) whole cell extracts of untransfected 293 cells, (2) whole cell extracts of 293 cells transfected with Cre, (3) whole cell extracts of 293 cells transfected with Dre, (4) whole cell extracts of 293 cells transfected with Flp.
Enzyme-linked Immunoabsorbent Assay
ELISA is a quantitative method used to determine the titer of the antibody using a serial dilution of antibody against Dre in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:1850.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Dre.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of Dre recombinase.
Host
Rabbit
Form
Liquid
Recommend Usage
ELISA (1:100)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In whole antiserum (0.05% sodium azide).
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of (1) whole cell extracts of untransfected 293 cells, (2) whole cell extracts of 293 cells transfected with Cre, (3) whole cell extracts of 293 cells transfected with Dre, (4) whole cell extracts of 293 cells transfected with Flp.Enzyme-linked Immunoabsorbent Assay
ELISA is a quantitative method used to determine the titer of the antibody using a serial dilution of antibody against Dre in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:1850.
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