ERAS polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of ERAS polyclonal antibody (Cat # PAB3129) in K-562 cell line lysates (35 ug/lane). ERAS (arrow) was detected using the purified polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human brain tissue reacted with ERAS polyclonal antibody (Cat # PAB3129) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Immunofluorescence
Immunofluorescence analysis of ERAS polyclonal antibody (Cat # PAB3129) in HeLa cells. Primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Flow Cytometry
Flow cytometric analysis of HeLa cells using ERAS polyclonal antibody (Cat # PAB3129)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ERAS.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human ERAS.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunofluorescence (1:10-50)
Immunohistochemistry (1:50)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of ERAS polyclonal antibody (Cat # PAB3129) in K-562 cell line lysates (35 ug/lane). ERAS (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human brain tissue reacted with ERAS polyclonal antibody (Cat # PAB3129) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Immunofluorescence
Immunofluorescence analysis of ERAS polyclonal antibody (Cat # PAB3129) in HeLa cells. Primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.Flow Cytometry
Flow cytometric analysis of HeLa cells using ERAS polyclonal antibody (Cat # PAB3129)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — ERAS
Entrez GeneID
3266Protein Accession#
NP_853510;Q7Z444Gene Name
ERAS
Gene Alias
HRAS2, HRASP, MGC126691, MGC126693
Gene Description
ES cell expressed Ras
Omim ID
300437Gene Ontology
HyperlinkOther Designations
OTTHUMP00000065857|small GTPase protein E-Ras|v-Ha-ras Harvey rat sarcoma viral oncogene homolog 2|v-Ha-ras Harvey rat sarcoma viral oncogene homolog pseudogene
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Interactome
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Publication Reference
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Human ERas gene has an upstream premature polyadenylation signal that results in a truncated, noncoding transcript.
Kameda T, Thomson JA.
Stem Cells 2005 Nov; 23(10):1535.
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Role of ERas in promoting tumour-like properties in mouse embryonic stem cells.
Takahashi K, Mitsui K, Yamanaka S.
Nature 2003 May; 423(6939):541.
Application：WB, Human, Mouse, CGR8, J1, MG1.19, RF8 cells.
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The human c-Ha-ras2 is a processed pseudogene inactivated by numerous base substitutions.
Miyoshi J, Kagimoto M, Soeda E, Sakaki Y.
Nucleic Acids Research 1984 Feb; 12(4):1821.
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Human ERas gene has an upstream premature polyadenylation signal that results in a truncated, noncoding transcript.
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