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RUNX1T1-ETO polyclonal antibody

  • Catalog # : PAB31285
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of RUNX1T1-ETO.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to human RUNX1T1-ETO fusion protein.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Whole antiserum
  • Recommend Usage:
  • ELISA (1:500)
    Western Blot (1:1000)
    ChIP (4 ul/CHIP)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Whole antiserum (0.05% sodium azide).
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • 3.
  • MEIS2 Is an Oncogenic Partner in AML1-ETO-Positive AML.
    Vegi NM, Klappacher J, Oswald F, Mulaw MA, Mandoli A, Thiel VN, Bamezai S, Feder K, Martens JH, Rawat VP, Mandal T, Quintanilla-Martinez L, Spiekermann K, Hiddemann W, Döhner K, Döhner H, Stunnenberg HG, Feuring-Buske M, Buske C.Cell Rep.2016 Jul 12;16(2):498-507.
  • Applications
  • ChIP
  • ChIP
  • ChIP assays were performed using Kasumi-1 cells. Sheared chromatin from 1.25 million cells and 4 ul of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. The figure shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.
  • ChIP-Seq
  • ChIP-Seq
  • The figure shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.
  • ELISA
  • ELISA
  • ELISA is a quantitative method used to determine the titer of the antibody using a serial dilution of antibody against RUNX1T1-ETO. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:32750.
  • Application Image
  • Gene Information
  • Entrez GeneID:
  • 861
  • Protein Accession#:
  • Q01196; Q06455
  • Gene Name:
  • RUNX1
  • Gene Alias:
  • AML1,AML1-EVI-1,AMLCR1,CBFA2,EVI-1,PEBP2aB
  • Gene Description:
  • runt-related transcription factor 1
  • Gene Summary:
  • Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • AML1-EVI-1 fusion protein,acute myeloid leukemia 1,aml1 oncogene,core-binding factor, runt domain, alpha subunit 2
  • Gene Information
  • Entrez GeneID:
  • 862
  • Protein Accession#:
  • Q01196; Q06455
  • Gene Name:
  • RUNX1T1
  • Gene Alias:
  • AML1T1,CBFA2T1,CDR,ETO,MGC2796,MTG8,MTG8b,ZMYND2
  • Gene Description:
  • runt-related transcription factor 1; translocated to, 1 (cyclin D-related)
  • Gene Summary:
  • The protein encoded by this gene is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Several transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • acute myelogenous leukemia 1 translocation 1 protein,acute myelogenous leukemia 1 translocation 1, cyclin-D related,core-binding factor, runt domain, alpha subunit 2; translocated to, 1; cyclin D-related,eight twenty one protein,myeloid translocation gene
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