RUNX1T1-ETO polyclonal antibody
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More Files
- More Functions
ChIP
ChIP assays were performed using Kasumi-1 cells. Sheared chromatin from 1.25 million cells and 4 ul of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. The figure shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.
ChIP-Seq
The figure shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.
Enzyme-linked Immunoabsorbent Assay
ELISA is a quantitative method used to determine the titer of the antibody using a serial dilution of antibody against RUNX1T1-ETO. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:32750.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RUNX1T1-ETO.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to human RUNX1T1-ETO fusion protein.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Whole antiserum
Recommend Usage
ELISA (1:500)
Western Blot (1:1000)
ChIP (4 ul/CHIP)
The optimal working dilution should be determined by the end user.Storage Buffer
In Whole antiserum (0.05% sodium azide).
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
ChIP assays were performed using Kasumi-1 cells. Sheared chromatin from 1.25 million cells and 4 ul of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. The figure shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.ChIP-Seq
The figure shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.Enzyme-linked Immunoabsorbent Assay
ELISA is a quantitative method used to determine the titer of the antibody using a serial dilution of antibody against RUNX1T1-ETO. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:32750. -
Gene Info — RUNX1
Entrez GeneID
861Protein Accession#
Q01196; Q06455Gene Name
RUNX1
Gene Alias
AML1, AML1-EVI-1, AMLCR1, CBFA2, EVI-1, PEBP2aB
Gene Description
runt-related transcription factor 1
Gene Ontology
HyperlinkGene Summary
Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
AML1-EVI-1 fusion protein|acute myeloid leukemia 1|aml1 oncogene|core-binding factor, runt domain, alpha subunit 2
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Gene Info — RUNX1T1
Entrez GeneID
862Protein Accession#
Q01196; Q06455Gene Name
RUNX1T1
Gene Alias
AML1T1, CBFA2T1, CDR, ETO, MGC2796, MTG8, MTG8b, ZMYND2
Gene Description
runt-related transcription factor 1; translocated to, 1 (cyclin D-related)
Omim ID
133435Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Several transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq
Other Designations
acute myelogenous leukemia 1 translocation 1 protein|acute myelogenous leukemia 1 translocation 1, cyclin-D related|core-binding factor, runt domain, alpha subunit 2; translocated to, 1; cyclin D-related|eight twenty one protein|myeloid translocation gene
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Interactome
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Pathway
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Disease
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Publication Reference
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Suppression of RUNX1/ETO oncogenic activity by a small molecule inhibitor of tetramerization.
Schanda J, Lee CW, Wohlan K, Müller-Kuller U, Kunkel H, Coco IQ, Stein S, Metz A, Koch J, Lausen J, Platzbecker U, Medyouf H, Gohlke H, Heuser M, Eder M, Grez M, Scherr M, Wichmann C.
Haematologica 2017 May; 102(5):e170.
Application：ChIP, Human, SKNO1 cells.
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The Hematopoietic Transcription Factors RUNX1 and ERG Prevent AML1-ETO Oncogene Overexpression and Onset of the Apoptosis Program in t(8;21) AMLs.
Mandoli A, Singh AA, Prange KH, Tijchon E, Oerlemans M, Dirks R, Ter Huurne M, Wierenga AT, Janssen-Megens EM, Berentsen K, Sharifi N, Kim B, Matarese F, Nguyen LN, Hubner NC, Rao NA, van den Akker E, Altucci L, Vellenga E, Stunnenberg HG, Martens JH.
Cell Reports 2016 Nov; 17(8):2087.
Application：ChIP-Seq, WB-Ce, WB-Tr, Human, Kasumi-1 cells.
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MEIS2 Is an Oncogenic Partner in AML1-ETO-Positive AML.
Vegi NM, Klappacher J, Oswald F, Mulaw MA, Mandoli A, Thiel VN, Bamezai S, Feder K, Martens JH, Rawat VP, Mandal T, Quintanilla-Martinez L, Spiekermann K, Hiddemann W, Dohner K, Döhner H, Stunnenberg HG, Feuring-Buske M, Buske C.
Cell Reports 2016 Jul; 16(2):498.
Application：ChIP, IP, WB-Tr, Human, HEK 293, Kasumi-1, SKNO-1 cells.
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Loss of TET2 in hematopoietic cells leads to DNA hypermethylation of active enhancers and induction of leukemogenesis.
Rasmussen KD, Jia G, Johansen JV, Pedersen MT, Rapin N, Bagger FO, Porse BT, Bernard OA, Christensen J, Helin K.
Genes & Development 2015 May; 29(9):910.
Application：ChIP, Mouse, AE cells.
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Immune evasion by oncogenic proteins of acute myeloid leukemia.
Elias S, Yamin R, Golomb L, Tsukerman P, Stanietsky-Kaynan N, Ben-Yehuda D, Mandelboim O.
Blood 2014 Mar; 123(10):1535.
Application：ChIP, Human, Kasumi-1 cells.
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ERG and FLI1 binding sites demarcate targets for aberrant epigenetic regulation by AML1-ETO in acute myeloid leukemia.
Martens JH, Mandoli A, Simmer F, Wierenga BJ, Saeed S, Singh AA, Altucci L, Vellenga E, Stunnenberg HG.
Blood 2012 Nov; 120(19):4038.
Application：ChIP-Seq, Human, SKNO-1 cells.
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Suppression of RUNX1/ETO oncogenic activity by a small molecule inhibitor of tetramerization.
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