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SAP30 polyclonal antibody

  • Catalog # : PAB31280
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against recombiant SAP30.
  • Immunogen:
  • Recombinant His-tag fusion protein corresponding to human SAP30.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • Western Blot (1:1000)
    ChIP (2-5 ug/CHIP)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide, 0.05% proclin 300).
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • ChIP
  • ChIP
  • ChIP assays were performed using HeLa cells. A titration of the antibody consisting of 1, 2 and 5 ug per ChIP experiment was analysed. IgG (1 ug/ IP) was used as negative IP control. QPCR was performed with primers for the EIF2S3 and BRCA1 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
  • ChIP-Seq
  • ChIP-Seq
  • ChIP was performed on sheared chromatin from 4 million HeLa cells using 5 ug antibody. The figure shows the enrichment along the complete sequence and a 1.5 Mb region of human chromosome 1 and in two genomic regions surrounding the BRCA1 and EIF2S3 genes on chromosome 17 and X.
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate) analysis of 20 ug nuclear extracts of HeLa cells.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 8819
  • Gene Name:
  • SAP30
  • Gene Alias:
  • -
  • Gene Description:
  • Sin3A-associated protein, 30kDa
  • Gene Summary:
  • Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP18, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This complex is active in deacetylating core histone octamers, but inactive in deacetylating nucleosomal histones. A pseudogene of this gene is located on chromosome 3. [provided by RefSeq
  • Other Designations:
  • Sin3 corepressor complex subunit SAP30,Sin3-associated polypeptide, 30kDa,histone deacetylase complex subunit SAP30
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