RARA polyclonal antibody
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More Files
- More Functions
ChIP
ChIP assays were performed using NB4 cells. Sheared chromatin from 6 million cells and 4 ul of antibody were used per ChIP experiment. QPCR was performed using primers specific for the TGM2, HMHA1, PRAM1 and H2B genes. The figure shows the relative occupancy, calculated as the ratio + control/background for which the second exon of the MB gene was used.
ChIP-Seq
The figure shows the results of the complete chromosome 19 and two 50 kb region surrounding the HMHA1 and PRAM1 genes, respectively.
Western Blot (Transfected lysate)
Western Blot (Cell lysate) analysis of (1) Negative control (vector only transfected HEK293 cells), and (2) HEK293 cells transfected with RARA construct.
Enzyme-linked Immunoabsorbent Assay
ELISA is a quantitative method used to determine the titer of the antibody using a serial dilution of antibody against human RARA. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2400.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RARA.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human RARA.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Whole antiserum
Recommend Usage
ELISA (1:50)
Western Blot (1:750)
ChIP/CHIP-seq (4 ug/CHIP)
The optimal working dilution should be determined by the end user.Storage Buffer
In Whole antiserum (0.05% sodium azide).
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
ChIP assays were performed using NB4 cells. Sheared chromatin from 6 million cells and 4 ul of antibody were used per ChIP experiment. QPCR was performed using primers specific for the TGM2, HMHA1, PRAM1 and H2B genes. The figure shows the relative occupancy, calculated as the ratio + control/background for which the second exon of the MB gene was used.ChIP-Seq
The figure shows the results of the complete chromosome 19 and two 50 kb region surrounding the HMHA1 and PRAM1 genes, respectively.Western Blot (Transfected lysate)
Western Blot (Cell lysate) analysis of (1) Negative control (vector only transfected HEK293 cells), and (2) HEK293 cells transfected with RARA construct.Enzyme-linked Immunoabsorbent Assay
ELISA is a quantitative method used to determine the titer of the antibody using a serial dilution of antibody against human RARA. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2400. -
Gene Info — RARA
Entrez GeneID
5914Protein Accession#
P10276Gene Name
RARA
Gene Alias
NR1B1, RAR
Gene Description
retinoic acid receptor, alpha
Omim ID
180240Gene Ontology
HyperlinkGene Summary
Retinoid signaling is transduced by 2 families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor (RXR; see MIM 180245), which form RXR/RAR heterodimers. In the absence of ligand, DNA-bound RXR/RARA represses transcription by recruiting the corepressors NCOR1 (MIM 600849), SMRT (NCOR2; MIM 600848), and histone deacetylase (see MIM 601241). When ligand binds to the complex, it induces a conformational change allowing the recruitment of coactivators, histone acetyltransferases (see MIM 603053), and the basic transcription machinery. Translocations that always involve rearrangement of the RARA gene are a cardinal feature of acute promyelocytic leukemia (APL; MIM 612376). The most frequent translocation is t(15,17)(q21;q22), which fuses the RARA gene with the PML gene (MIM 102578) (Vitoux et al., 2007 [PubMed 17468032]).[supplied by OMIM
Other Designations
OTTHUMP00000164454|OTTHUMP00000164456|Retinoic acid receptor, alpha polypeptide|nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form
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Interactome
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Pathway
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Disease
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Publication Reference
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Elevated Fibroblast growth factor 21 (FGF21) in obese, insulin resistant states is normalised by the synthetic retinoid Fenretinide in mice.
Morrice N. et al.
Scientific Reports 2017 Mar; 7:43782.
Application：ChIP, Mouse, Mouse liver.
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The oncofusion protein FUS-ERG targets key hematopoietic regulators and modulates the all-trans retinoic acid signaling pathway in t(16;21) acute myeloid leukemia.
Sotoca AM, Prange KH, Reijnders B, Mandoli A, Nguyen LN, Stunnenberg HG, Martens JH.
Oncogene 2016 Apr; 35(15):1965.
Application：ChIP, Human, TSU-1621-MT cells.
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Retinoic acid is essential for Th1 cell lineage stability and prevents transition to a Th17 cell program.
Brown CC, Esterhazy D, Sarde A, London M, Pullabhatla V, Osma-Garcia I, Al-Bader R, Ortiz C, Elgueta R, Arno M, de Rinaldis E, Mucida D, Lord GM, Noelle RJ.
Immunity 2015 Mar; 42(3):499.
Application：ChIP-Seq, Mouse, Mouse T cells.
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PML-RARalpha/RXR Alters the Epigenetic Landscape in Acute Promyelocytic Leukemia.
Martens JH, Brinkman AB, Simmer F, Francoijs KJ, Nebbioso A, Ferrara F, Altucci L, Stunnenberg HG.
Cancer Cell 2010 Feb; 17(2):173.
Application：ChIP-Seq, Human, NB4 cells.
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Elevated Fibroblast growth factor 21 (FGF21) in obese, insulin resistant states is normalised by the synthetic retinoid Fenretinide in mice.
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