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RARA polyclonal antibody

  • Catalog # : PAB31279
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of RARA.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human RARA.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Whole antiserum
  • Recommend Usage:
  • ELISA (1:50)
    Western Blot (1:750)
    ChIP/CHIP-seq (4 ug/CHIP)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Whole antiserum (0.05% sodium azide).
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • ChIP
  • ChIP
  • ChIP assays were performed using NB4 cells. Sheared chromatin from 6 million cells and 4 ul of antibody were used per ChIP experiment. QPCR was performed using primers specific for the TGM2, HMHA1, PRAM1 and H2B genes. The figure shows the relative occupancy, calculated as the ratio + control/background for which the second exon of the MB gene was used.
  • ChIP-Seq
  • ChIP-Seq
  • The figure shows the results of the complete chromosome 19 and two 50 kb region surrounding the HMHA1 and PRAM1 genes, respectively.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot (Cell lysate) analysis of (1) Negative control (vector only transfected HEK293 cells), and (2) HEK293 cells transfected with RARA construct.
  • ELISA
  • ELISA
  • ELISA is a quantitative method used to determine the titer of the antibody using a serial dilution of antibody against human RARA. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2400.
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 5914
  • Gene Name:
  • RARA
  • Gene Alias:
  • NR1B1,RAR
  • Gene Description:
  • retinoic acid receptor, alpha
  • Gene Summary:
  • Retinoid signaling is transduced by 2 families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor (RXR; see MIM 180245), which form RXR/RAR heterodimers. In the absence of ligand, DNA-bound RXR/RARA represses transcription by recruiting the corepressors NCOR1 (MIM 600849), SMRT (NCOR2; MIM 600848), and histone deacetylase (see MIM 601241). When ligand binds to the complex, it induces a conformational change allowing the recruitment of coactivators, histone acetyltransferases (see MIM 603053), and the basic transcription machinery. Translocations that always involve rearrangement of the RARA gene are a cardinal feature of acute promyelocytic leukemia (APL; MIM 612376). The most frequent translocation is t(15,17)(q21;q22), which fuses the RARA gene with the PML gene (MIM 102578) (Vitoux et al., 2007 [PubMed 17468032]).[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000164454,OTTHUMP00000164456,Retinoic acid receptor, alpha polypeptide,nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form
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