Histone H4 pan polyclonal antibody
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More Files
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ChIP
ChIP assays were performed using human HeLa cells. A titration consisting of 0.5, 1, 2 and 5 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoters of the active GAPDH and c-fos genes, and for the inactive MYOD1 gene and the Sat2 satellite repeat. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of (1) 25 ug whole cell extracts of HeLa cells, (2) 15 ug histone extracts of HeLa cells, (3) 1 ug of recombinant histone H2A, (4) 1 ug of recombinant histone H2B, (5) 1 ug of recombinant histone H3, and (6) 1 ug of recombinant histone H4.
Immunofluorescence
Immunofluorescent staining of Hela cell line with antibody followed by an anti-rabbit antibody conjugated to Alexa488 (middle). The left panel shows staining of the nuclei with DAPI. A merge of the two stainings (right).
Enzyme-linked Immunoabsorbent Assay
ELISA is a quantitative method used to determine the titer of the antibody using a serial dilution of antibody against Histone H4 in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:3000.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Histone H4 pan.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to unmodified sequences at the central part of human histone H4 pan.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Recommend Usage
ELISA (1:100)
Western Blot (1:1000)
ChIP (1-2 ug/CHIP)
Immunofluorescence (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide, 0.05% proclin 300).
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
ChIP assays were performed using human HeLa cells. A titration consisting of 0.5, 1, 2 and 5 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoters of the active GAPDH and c-fos genes, and for the inactive MYOD1 gene and the Sat2 satellite repeat. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of (1) 25 ug whole cell extracts of HeLa cells, (2) 15 ug histone extracts of HeLa cells, (3) 1 ug of recombinant histone H2A, (4) 1 ug of recombinant histone H2B, (5) 1 ug of recombinant histone H3, and (6) 1 ug of recombinant histone H4.Immunofluorescence
Immunofluorescent staining of Hela cell line with antibody followed by an anti-rabbit antibody conjugated to Alexa488 (middle). The left panel shows staining of the nuclei with DAPI. A merge of the two stainings (right).Enzyme-linked Immunoabsorbent Assay
ELISA is a quantitative method used to determine the titer of the antibody using a serial dilution of antibody against Histone H4 in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:3000. -
Gene Info — HIST1H4A
Entrez GeneID
8359Protein Accession#
P62805Gene Name
HIST1H4A
Gene Alias
H4/a, H4FA
Gene Description
histone cluster 1, H4a
Omim ID
602822Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq
Other Designations
H4 histone family, member A|histone 1, H4a
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Interactome
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Pathway
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Publication Reference
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Passaging techniques and ROCK inhibitor exert reversible effects on morphology and pluripotency marker gene expression of human embryonic stem cell lines.
Holm F, Nikdin H, Kjartansdottir KR, Gaudenzi G, Fried K, Aspenström P, Hermanson O, Bergström-Tengzelius R.
Stem Cells and Development 2013 Jul; 22(13):1883.
Application：ChIP, Human, Human embryonic stem cells.
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Passaging techniques and ROCK inhibitor exert reversible effects on morphology and pluripotency marker gene expression of human embryonic stem cell lines.
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