Histone H3 (K9me3) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of 20 ug histone extracts of HeLa cells.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Histone H3 (K9me3).
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to Histone H3, trimethylated at lysine 9.
Host
Rabbit
Reactivity
Fruit fly, Human, Mouse
Form
Liquid
Purification
Whole antiserum
Recommend Usage
Western Blot (1:750)
ChIP (1-5 ul/CHIP)
The optimal working dilution should be determined by the end user.Storage Buffer
In Whole antiserum (0.05% sodium azide).
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of 20 ug histone extracts of HeLa cells. -
Gene Info — HIST1H3A
Entrez GeneID
8350Protein Accession#
P68431Gene Name
HIST1H3A
Gene Alias
H3/A, H3FA
Gene Description
histone cluster 1, H3a
Omim ID
602810Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
Other Designations
H3 histone family, member A|histone 1, H3a
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Interactome
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Pathway
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Publication Reference
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Spatiotemporal control of estrogen-responsive transcription in ERα-positive breast cancer cells.
P-Y Hsu, H-K Hsu, T-H Hsiao, Z Ye, E Wang, A L Profit, I Jatoi, Y Chen, N B Kirma, V X Jin, Z D Sharp and T H-M Huang.
Oncogene 2016 May; 35(18):2379.
Application：ChIP, Human, MCF-7 cells.
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Spatiotemporal control of estrogen-responsive transcription in ERα-positive breast cancer cells.
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