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Last updated: 2017/10/15

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Histone H2B pan polyclonal antibody

  • Catalog # : PAB31259
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Histone H2B pan.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to unmodified sequence at C-terminus of human histone H2B pan.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Recommend Usage:
  • ELISA (1:500)
    ChIP (2 ug/CHIP)
    Dot Blot (1:20000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide, 0.05% proclin 300).
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • ChIP
  • ChIP
  • ChIP assays were performed using human HeLa cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH promoter and for the inactive MYOD gene. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
  • ELISA
  • ELISA
  • ELISA is a quantitative method used to determine the titer of the antibody using a serial dilution of crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:14950.
  • Dot Blot
  • Dot Blot
  • Cross reactivity test using the Histone H2B (pan) antibody.
    Dot Blot analysis was performed with the peptide used for immunization of the rabbit and other peptides containing unmodified sequences of different histones. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20000 and shows a high specificity of the antibody for the H2B peptide.
  • Application Image
  • Gene Information
  • Entrez GeneID:
  • 8347
  • Gene Name:
  • HIST1H2BC
  • Gene Alias:
  • H2B.1,H2B/l,H2BFL,MGC104246,dJ221C16.3
  • Gene Description:
  • histone cluster 1, H2bc
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq
  • Other Designations:
  • H2B histone family, member L,OTTHUMP00000016141,histone 1, H2bc
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