ALDH1A1 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot analysis of ALDH1A1 (arrow) using rabbit ALDH1A1 polyclonal antibody (Cat # PAB3123). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALDH1A1 gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with ALDH1A1 polyclonal antibody (Cat # PAB3123) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Immunofluorescence
Immunofluorescence analysis of ALDH1A1 polyclonal antibody (Cat # PAB3123) in HeLa cells. Primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Flow Cytometry
Flow cytometric analysis of HepG2 cells using ALDH1A1 polyclonal antibody (Cat # PAB3123)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ALDH1A1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human ALDH1A1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunofluorescence (1:10-50)
Immunohistochemistry (1:50)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of ALDH1A1 (arrow) using rabbit ALDH1A1 polyclonal antibody (Cat # PAB3123). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALDH1A1 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with ALDH1A1 polyclonal antibody (Cat # PAB3123) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Immunofluorescence
Immunofluorescence analysis of ALDH1A1 polyclonal antibody (Cat # PAB3123) in HeLa cells. Primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.Flow Cytometry
Flow cytometric analysis of HepG2 cells using ALDH1A1 polyclonal antibody (Cat # PAB3123)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — ALDH1A1
Entrez GeneID
216Protein Accession#
NP_000680;P00352Gene Name
ALDH1A1
Gene Alias
ALDC, ALDH-E1, ALDH1, ALDH11, MGC2318, PUMB1, RALDH1
Gene Description
aldehyde dehydrogenase 1 family, member A1
Gene Ontology
HyperlinkGene Summary
This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a cytosolic isoform, which has a high affinity for aldehydes. [provided by RefSeq
Other Designations
ALDH class 1|acetaldehyde dehydrogenase 1|aldehyde dehydrogenase 1, soluble|aldehyde dehydrogenase 1A1|aldehyde dehydrogenase, liver cytosolic|retinal dehydrogenase 1|retinaldehyde dehydrogenase 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Association of ALDH1 promoter polymorphisms with alcohol-related phenotypes in Trinidad and Tobago.
Moore S, Montane-Jaime K, Shafe S, Joseph R, Crooks H, Carr LG, Ehlers CL.
Journal of Studies on Alcohol and Drugs 2007 Mar; 68(2):192.
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Identification of 3-deoxyglucosone dehydrogenase as aldehyde dehydrogenase 1A1 (retinaldehyde dehydrogenase 1).
Collard F, Vertommen D, Fortpied J, Duester G, Van Schaftingen E.
Biochimie 2007 Mar; 89(3):369.
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Association of ALDH1 promoter polymorphisms with alcohol-related phenotypes in Trinidad and Tobago.
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