MIPEP polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot analysis of MIPEP (arrow) using rabbit MIPEP polyclonal antibody (Cat # PAB3114). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MIPEP gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MIPEP polyclonal antibody (Cat # PAB3114) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MIPEP.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human MIPEP.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of MIPEP (arrow) using rabbit MIPEP polyclonal antibody (Cat # PAB3114). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MIPEP gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MIPEP polyclonal antibody (Cat # PAB3114) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — MIPEP
Entrez GeneID
4285Protein Accession#
NP_005923;Q99797Gene Name
MIPEP
Gene Alias
HMIP, MIP
Gene Description
mitochondrial intermediate peptidase
Omim ID
602241Gene Ontology
HyperlinkGene Summary
The product of this gene performs the final step in processing a specific class of nuclear-encoded proteins targeted to the mitochondrial matrix or inner membrane. This protein is primarily involved in the maturation of oxidative phosphorylation (OXPHOS)-related proteins. This gene may contribute to the functional effects of frataxin deficiency and the clinical manifestations of Friedreich ataxia. [provided by RefSeq
Other Designations
OTTHUMP00000018121|OTTHUMP00000042292
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Interactome
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Publication Reference
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Cloning, expression, and chromosomal assignment of the human mitochondrial intermediate peptidase gene (MIPEP).
Chew A, Buck EA, Peretz S, Sirugo G, Rinaldo P, Isaya G.
Genomics 1997 Mar; 40(3):493.
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Cloning, expression, and chromosomal assignment of the human mitochondrial intermediate peptidase gene (MIPEP).
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