KIF2A polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human KIF2A.
Immunogen
Recombinant protein corresponding to amino acids 35-144 of human KIF2A.
Sequence
DNESVTVEWIENGDTKGKEIDLESIFSLNPDLVPDEEIEPSPETPPPPASSAKVNKIVKNRRTVASIKNDPPSRDNRVVGSARARPSQFPEQSSSAQQNGSVSDISPVQA
Host
Rabbit
Reactivity
Human, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:20-1:50)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C for short term storage. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of (1) human cell line RT-4, and (2) human cell line U-251MG sp.Western Blot
Western Blot analysis of (1) NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), and (2) NBT-II cell lysate (Rat Wistar bladder tumour cells).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human testis shows nuclear positivity in cells of seminiferus ducts. -
Gene Info — KIF2A
Entrez GeneID
3796Protein Accession#
O00139Gene Name
KIF2A
Gene Alias
HK2, KIF2
Gene Description
kinesin heavy chain member 2A
Omim ID
602591Gene Ontology
HyperlinkGene Summary
Kinesins, such as KIF2, are microtubule-associated motor proteins. For background information on kinesins, see MIM 148760.[supplied by OMIM
Other Designations
Kinesin, heavy chain, 2|kinesin heavy chain member 2
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Interactome
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Disease
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Publication Reference
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Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model.
Kato BS, Nicholson G, Neiman M, Rantalainen M, Holmes CC, Barrett A, Uhlén M, Nilsson P, Spector TD, Schwenk JM.
Proteome Science 2011 Nov; 9:73.
Application:Array, Recombinant protein.
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Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods.
Jakobsen L, Vanselow K, Skogs M, Toyoda Y, Lundberg E, Poser I, Falkenby LG, Bennetzen M, Westendorf J, Nigg EA, Uhlen M, Hyman AA, Andersen JS.
The EMBO Journal 2011 Apr; 30(8):1520.
Application:IF, Human, U-2 OS cells.
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Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model.
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