WNT10A polyclonal antibody

Catalog # PAB31064

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Size:100 uL
Price: USD $ 428.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
Western Blot (Transfected lysate)
Application

Western Blot (Transfected lysate)

Western Blot (Cell lysate) analysis of (1) Negative control (vector only transfected HEK293T lysate), and (2) wNT10A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical staining of human skin shows weak cytoplasmic positivity in epidermal cells.

  • Specification

    Product Description

    Rabbit polyclonal antibody raised against partial recombinant human WNT10A.

    Immunogen

    Recombinant protein corresponding to human WNT10A.

    Sequence

    YESPIFSRGFRESAFAYAIAAAGVVHAVSNACALGKLKACGCDASRRGDEEAFRRKLHRLQLDALQRGKGLSHGVPEHPALPTASPGLQDSWEWGGCSPDMGFGERFSKDFLDSR

    Host

    Rabbit

    Reactivity

    Human

    Form

    Liquid

    Purification

    Antigen affinity purification

    Isotype

    IgG

    Recommend Usage

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-200)
    Western Blot (1:100-250)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).

    Storage Instruction

    Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Transfected lysate)

    Western Blot (Cell lysate) analysis of (1) Negative control (vector only transfected HEK293T lysate), and (2) wNT10A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemical staining of human skin shows weak cytoplasmic positivity in epidermal cells.
  • Gene Info — WNT10A

    Entrez GeneID

    80326

    Protein Accession#

    Q9GZT5

    Gene Name

    WNT10A

    Gene Alias

    FLJ14301

    Gene Description

    wingless-type MMTV integration site family, member 10A

    Omim ID

    257980 606268

    Gene Ontology

    Hyperlink

    Gene Summary

    The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is strongly expressed in the cell lines of promyelocytic leukemia and Burkitt's lymphoma. In addition, it and another family member, the WNT6 gene, are strongly coexpressed in colorectal cancer cell lines. The gene overexpression may play key roles in carcinogenesis through activation of the WNT-beta-catenin-TCF signaling pathway. This gene and the WNT6 gene are clustered in the chromosome 2q35 region. [provided by RefSeq

    Other Designations

    -

  • Interactome
  • Pathway
  • Disease
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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