PSMA5 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of PSMA5 polyclonal antibody (Cat # PAB3101) in HL-60 cell line lysates (35 ug/lane). PSMA5 (arrow) was detected using the purified polyclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of PSMA5 (arrow) using rabbit PSMA5 polyclonal antibody (Cat # PAB3101). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PSMA5 gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with PSMA5 polyclonal antibody (Cat # PAB3101) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PSMA5.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human PSMA5.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of PSMA5 polyclonal antibody (Cat # PAB3101) in HL-60 cell line lysates (35 ug/lane). PSMA5 (arrow) was detected using the purified polyclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of PSMA5 (arrow) using rabbit PSMA5 polyclonal antibody (Cat # PAB3101). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PSMA5 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with PSMA5 polyclonal antibody (Cat # PAB3101) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — PSMA5
Entrez GeneID
5686Protein Accession#
NP_002781;P28066Gene Name
PSMA5
Gene Alias
MGC117302, MGC125802, MGC125803, MGC125804, PSC5, ZETA
Gene Description
proteasome (prosome, macropain) subunit, alpha type, 5
Omim ID
176844Gene Ontology
HyperlinkGene Summary
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. [provided by RefSeq
Other Designations
OTTHUMP00000013792|macropain subunit zeta|macropain zeta chain|multicatalytic endopeptidase complex zeta chain|proteasome alpha 5 subunit|proteasome component 5|proteasome subunit zeta|proteasome zeta chain
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Interactome
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Pathway
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