PRAF2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human PRAF2.
Immunogen
Recombinant protein corresponding to human PRAF2.
Sequence
MSEVRLPPLRALDDFVLGSARLAAPDPCDPQRWCHRVINN
Host
Rabbit
Reactivity
Human, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:20-1:50)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: NIH-3T3 cell lysate (mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Wistar rat bladder tumor cells) and Lane 3: PC12 cell lysate (pheochromocytoma of rat adrenal medulla) with PRAF2 polyclonal antibody (Cat # PAB31004).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebellum with PRAF2 polyclonal antibody (Cat # PAB31004) shows strong cytoplasmic positivity in Purkinje cells. -
Gene Info — PRAF2
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Interactome
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Publication Reference
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Expression profile of PRAF2 in the human brain and enrichment in synaptic vesicles.
Koomoa DL, Go RC, Wester K, Bachmann AS.
Neuroscience Letters 2008 May; 436(2):171.
Application:IHC-P, WB-Ti, Human, Rat, Human brains, Rat brains.
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Expression profile of PRAF2 in the human brain and enrichment in synaptic vesicles.
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