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Last updated: 2016/12/4
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SPRED1 polyclonal antibody

  • Catalog # : PAB3100
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of SPRED1.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human SPRED1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Recommend Usage:
  • Western Blot (1:1000)
    Immunohistochemistry (1:10-50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of SPRED1 polyclonal antibody (Cat # PAB3100) (arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SPRED1 gene (Lane 2) (Origene Technologies).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human brain tissue reacted with SPRED1 polyclonal antibody (Cat # PAB3100) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Protein Accession#:
  • NP_689807;Q7Z699
  • Gene Name:
  • SPRED1
  • Gene Alias:
  • FLJ33903,NFLS
  • Gene Description:
  • sprouty-related, EVH1 domain containing 1
  • Gene Summary:
  • The protein encoded by this gene is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. The encoded protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). [provided by RefSeq
  • Other Designations:
  • EVH1/Sprouty domain containing protein,sprouty-related protein 1 with EVH-1 domain,suppressor of Ras/MAPK activation
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