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Last updated: 2016/10/23
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CDCA8 polyclonal antibody

  • Catalog # : PAB3096
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of CDCA8.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to internal region of human CDCA8.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Recommend Usage:
  • Western Blot (1:1000)
    Immunohistochemistry (1:10-50)
    Flow cytometry (1:10-50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of CDCA8 polyclonal antibody (Cat # PAB3096) in HL-60 cell line lysates (35 ug/lane). CDCA8 (arrow) was detected using the purified polyclonal antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CDCA8 polyclonal antibody (Cat # PAB3096) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HL-60 cells using CDCA8 polyclonal antibody (Cat # PAB3096)(bottom histogram) compared to a negative control cell (top histogram).
    FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Protein Accession#:
  • NP_060571;Q53HL2
  • Gene Name:
  • CDCA8
  • Gene Alias:
  • BOR,BOREALIN,FLJ10468,FLJ12042
  • Gene Description:
  • cell division cycle associated 8
  • Gene Summary:
  • CDCA8 is a component of a chromosomal passenger complex required for stability of the bipolar mitotic spindle (Gassmann et al., 2004 [PubMed 15249581]).[supplied by OMIM
  • Other Designations:
  • Dasra B,OTTHUMP00000004514,OTTHUMP00000004515
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