ELN polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human ELN.
Immunogen
Recombinant protein corresponding to human ELN.
Sequence
VKPGKVPGVGLPGVYPGGVLPGARFPGVGVLPGVPTGAGVKPKAPGVGGAFAGIPGVGPFGGPQPGVPLGYPIKAPKLPGGYGLPYTTGKLPYGYGPGGVAGAAGKAGYPTGTGVGPQAAAAAAAKAAAKFGAGA
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human skin with ELN polyclonal antibody (Cat # PAB30898) shows distinct positivity in extracellular fibers. -
Gene Info — ELN
Entrez GeneID
2006Protein Accession#
P15502Gene Name
ELN
Gene Alias
FLJ38671, FLJ43523, SVAS, WBS, WS
Gene Description
elastin
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is one of the two components of elastic fibers. The encoded protein is rich in hydrophobic amino acids such as glycine and proline, which form mobile hydrophobic regions bounded by crosslinks between lysine residues. Deletions and mutations in this gene are associated with supravalvular aortic stenosis (SVAS) and autosomal dominant cutis laxa. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000196667|tropoelastin
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Interactome
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Disease
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Publication Reference
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Elastogenesis at the onset of human cardiac valve development.
Votteler M, Berrio DA, Horke A, Sabatier L, Reinhardt DP, Nsair A, Aikawa E, Schenke-Layland K.
Development 2013 Jun; 140(11):2345.
Application:IF, IHC-P, Human, Human fetal semilunar valve leaflet section.
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Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Charlotte Stadler, Elton Rexhepaj, Vasanth R Singan, Robert F Murphy, Rainer Pepperkok, Mathias Uhlén, Jeremy C Simpson, Emma Lundberg.
Nature Methods 2013 Apr; 10(4):315.
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Elastogenesis at the onset of human cardiac valve development.
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